Binding constants determination of cyclodextrin inclusion complexes by affinity capillary electrophoresis. How to overcome the limitations induced by the UV-detector?

•Algorithmic treatment and competitive experiments extend the applicability of the ACE-UV.•Binding of strong, transparent and/or neutral substrates with β-CD can be determined.•Determining the migration times at peak start was relevant for classical linearization.•Global algorithmic treatment estimates the dilution of the sample molecules in capillary.