Usually overlooked problems related with measurements of high-heat effects using power compensation isothermal titration calorimetry

This research paper deals with the problems associated with power compensation isothermal titration calorimetry. It is demonstrated that at processes, accompanied by large heat effects or/and slow kinetic processes, it is possible to obtain different values of enthalpies with different instruments and different setting parameters. Dynamic power compensation mode in modern calorimeters could namely lead to overcompensation of the heat effects, giving results which are strongly dependent on the chosen experimental parameters. In order to avoid these problems with modern instruments, first the check of a raw signal is proposed and in the case where the switching effect is observed, the experiments have to be carried out with low or disabled power compensation. In this way, the duration of experiments will be longer and the measuring range will be narrower, but the experimental results will be correct.