[Effect of Bmi-1 Silencing on the Proliferation of K562/ADM Cells in Vitro and in Vivo].

OBJECTIVE:To investigate the effect of Bmi-1 gene silencing on the proliferation of K562/ADM cells in vitro and in vivo and to explore its possible molecular mechanism.METHODS:The small interference RNA (siRNA) sequences of Bmi-1 were transfected into K562/ADM cells for decreasing the expression of Bmi-1. The effect of Bmi-1 silencing on the proliferation of K562/ADM cells in vitro and in vivo was detected by using MTT method, cell colony-forming test and tumoriganicity of nude mice; the expression of Bmi-1, PTEN and PAKT proteins was detected by Wertern blot. The immunohistochemistry assay was used to analyze the expression of Bmi-1 and Ki-67.RESULTS:The Bmi-1 silencing could significantly inhibit the proliferation activity of K562/ADM cells, the cell colony-forming ability and tumorigenicity were reduced. LY294002 decreased p-AKT expression, cell colony-forming ability and tumorigenicity. Bpv reduced the PTEN expression but increased p-AKT expression and restored the colony-forming ability and tumorigenesis of K562/ADM-S1-Bpv cells. Bmi-1-siRNA dramatically suppressed the Bmi-1 and Ki-67 protein levels in xenograft tumor tissue.CONCLUSION:Bmi-1 can mediate the proliferation of K562/ADM cell, the PTEN/p-AKT might be involved in this pathway.