Synthesis of Mn3O4 nano complex using aqueous extract of Helianthus annuus seed cake and its effect on biological growth of Vigna radiata

Manganese Oxide complex (MnO3O4) nanoparticles were successfully synthesized by bio-synthesis method using Manganese Acetate as the precursor, mediated by aqueous extract of Helianthus annuus seed cake. The XRD pattern confirms the crystalline nature with tetragonal phase having the average particle size 57 nm. FTIR analysis reveals the presence of Manganese oxide and the absorption peak around 650 nm and 613 nm is assigned to Mn-O stretching. From UV analysis the band gap energy is calculated as 1.47eV. SEM and TEM analysis of the specimen showed, the distribution of nanoparticles is spherical in the range of 10 nm to 70 nm. VSM studies confirm the soft magnetic nature of the sample. The synthesized MnO nanoparticles were incorporated to the sandy loam soil to check the biological growth of Vigna Radiata (Green gram) .Growth attributes measured were plant height, number of leaves, shoot length etc. Germination parameters were calculated based on the observations by POT studies. Harvested plant samples were tested for its phytochemical and biochemical compounds. The results showed an increasing trend of total chlorophyll content and decreased protein content in the MnO treated sample with reference to the untreated plant. The manganese deficiency increased the root surface area due to increased consumption, reduced the amount of manganese translocation caused diminished shoot growth, and yielded an increased root ratio.