A 6-nm ultra-photostable DNA FluoroCube for fluorescence imaging
Nature Methods(2020)
摘要
Photobleaching limits extended imaging of fluorescent biological samples. We developed DNA-based ‘FluoroCubes’ that are similar in size to the green fluorescent protein, have single-point attachment to proteins, have a ~54-fold higher photobleaching lifetime and emit ~43-fold more photons than single organic dyes. We demonstrate that DNA FluoroCubes provide outstanding tools for single-molecule imaging, allowing the tracking of single motor proteins for >800 steps with nanometer precision.
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关键词
DNA,Single-molecule biophysics,Life Sciences,general,Biological Techniques,Biological Microscopy,Biomedical Engineering/Biotechnology,Bioinformatics,Proteomics
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