Comparative impact of platelet rich plasma and transforming growth factor- on chondrogenic differentiation of human adipose derived stem cells

Introduction: Transforming growth factor-beta (TGF-beta) is known as standard chondrogenic differentiation agent, even though it comes with undesirable side effects such as early hypertrophic maturation, mineralization, and secretion of inflammatory/angiogenic factors. On the other hand, platelet-rich plasma (PRP) is found to have a chondrogenic impact on mesenchymal stem cell proliferation and differentiation, with no considerable side effects. Therefore, we compared chondrogenic impact of TGF-beta and PRP on adipose-derived stem cells (ADSCs), to see if PRP could be introduced as an alternative to TGF-beta. Methods: Differentiation of ADSCs was monitored using a couple of methods including glycosaminoglycan production, miRNAs expression, vascular endothelial growth factor (VEGF)/ tumor necrosis factor alpha (TNFa) secretion, alkaline phosphatase (ALP) and calcium content assays. Results: Accordingly, the treatment of differentiating cells with 5% (v/v) PRP resulted in higher glycosaminoglycan production, enhanced SOX9 transcription, and lowered TNFa and VEGF secretion compared to the control and TGF-beta groups. Besides, the application of PRP to the media up-regulated miR-146a and miR-199a in early and late stages of chondrogenesis, respectively. Conclusion: PRP induces in vitro chondrogenesis, as well as TGF-beta with lesser inflammatory and hypertrophic side effects.