Methods of Evaluating the Efficiency of CRISPR/Cas Genome Editing
Molecular Biology(2019)
摘要
— The CRISPR/Cas system is currently widely used for genome editing. The procedure of genome editing includes two necessary steps: (i) searching for the most effective guide RNA, and (ii) analyzing clones for presence of the desired mutation. This review presents the methods used to assess the efficiency of the CRISPR/Cas system and to confirm mutation in the target locus and discusses their advantages and disadvantages. It aims to provide information that could help researchers to choose a technique most appropriate for their specific tasks and available resources.
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关键词
CRISPR/Сas9,NGS,droplet digital PCR,RFLP,T7E1,SURVEYOR,TIDE,IDAA,pDR/FACS,ENIT,SNP,HRMA,SSCP,cbPCR,DHPLC,LDR
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