Rapid in situ analysis of L-histidine and -lactose in dietary supplements by fingerprint peaks using terahertz frequency-domain spectroscopy

A simple, green and nondestructive method based on terahertz fingerprint peaks has been developed for rapid in situ analysis of L-histidine and alpha-lactose in dietary supplements. Fingerprint absorption peaks of L-histidine and alpha-lactose located at 0.77 and 0.53 THz could be directly used for identification and quantitation of these analytes in commercial dietary supplements. Compared with the partial least squares regression model (PLSR), the linear least squares regression (LLSR) method based on peak areas presented better performance, with the linear correlation coefficients of 0.9899 and 0.9910 for L-histidine and alpha-lactose, respectively. Furthermore, analysis time per sample can be shortened to less than 1 min due to the narrower spectral acquisition region. The accuracies were 94.8-110% and 98.9-110%, comparable to those of ion chromatography for L-hisddine and high-performance liquid chromatography for alpha-lactose. The results presented great potential of the developed method for rapid in situ analysis of nutrients in dietary supplements.