Analytical Evaluation of the Microarray-Based FluChip-8G Influenza A+B Assay.

Amber W Taylor,Erica D Dawson,Rebecca H Blair,James E Johnson,Amelia H Slinskey,Andrew W Smolak,Evan Toth,Kyle Liikanen,Robert S Stoughton,Catherine Smith,Sarah Talbot,Kathy L Rowlen

Journal of Virological Methods（2019）

引用 6|浏览15

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摘要

•Multiplex RT-PCR microarray assay for influenza typing and subtyping was evaluated.•Limits of detection were ∼200 genome copies/reaction, depending on strain.•Broad analytical reactivity over seasonal/non-seasonal strains was demonstrated.•No cross-reactivity with non-influenza organisms was observed.•Three-site inter-laboratory reproducibility was 98.5%.

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CI,LCL,UCL,PCM,LOD

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