L-Tryptophan represses persister formation via inhibiting bacterial motility and promoting antibiotics absorption.
FUTURE MICROBIOLOGY(2019)
摘要
Aim: The bacterial persisters have emerged as a huge threat to human health. Here, we investigated the role of L-tryptophan in bacterial persister killing by aminoglycoside antibiotics (AGs). Materials & methods: The relevance to the antibiotic susceptibility of Escherichia coli including transcriptional sequencing, gene expression, intracellular ATP, Nicotinamide adenine dinucleotide (NAD/NADH), reactive oxygen species and membrane depolarization were determined. Results & conclusion: We found that exogenous L-tryptophan efficiently inhibited AGs-enabled persisters. The flagellar genes were almost significantly downregulated. Besides, the AGs uptake was obviously increased as the result of elevation in proton motive force (PMF) in response to L-tryptophan-mediated NADH production. Taken together, these data supported a novel role of L-tryptophan in eradicating AGs persisters against E. coli.
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关键词
aminoglycosides antibiotics,antibiotic susceptibility,bacterial motility,Escherichia coli,L-tryptophan,NADH,persister,proton motive force (PMF),reactive oxygen species (ROS),tricarboxylic acid (TCA) cycle
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