Antimicrobial profile of Enterobacteriaceae and Vibrionaceae isolated from Cerastoderma edule.

Event Abstract Back to Event Antimicrobial profile of Enterobacteriaceae and Vibrionaceae isolated from Cerastoderma edule. Joana Ribeiro1, Pedro Pires1, Cátia F. Costa1, Teresa M. Baptista1, Americo Rodrigues1, Alberto Quesada2 and Maria J. Campos1* 1 Centro de Ciências do Mar e do Ambiente (IPLeiria), ESTM - Polytechnic Institute of Leiria, Portugal 2 Facultad de Veterinaria, Universidad de Extremadura, Departamento de Bioquímica, Biología Molecular y genética, Spain Cockles are a very popular shellfish food product in Portugal. Since cockles are filter feeders, they present the potential to accumulate great numbers of microorganisms including pathogens which can constitute a substantial food hazard when consumed raw or undercooked. Enterobacteria and autochthonous marine bacteria as Vibrionaceae present in this type of products may be very important due to its antimicrobial resistance. The aim of this study was to investigate the presence of antimicrobial resistance bacteria and its molecular determinants in cockle (Cerastoderma edule) harvested during November 2016 in Óbidos Lagoon, Óbidos, a shallow coastal lagoon in Portugal. Bacteria were isolated in selective medium for Enterobacteriaceae and Vibrionaceae containing the antimicrobials cefotaxime, ciprofloxacin and imipenem. Colonies with morphological distinct characteristics were selected from each media for further studies. The presence of genes qnrA, qnrB qnrS, qnrC, qnrD, aac(6’)-ib- cr, qepA, bla CTX-M , bla SHV , bla TEM , bla VIM , bla IMP-5 , bla KPC-2, bla NDM-1 , bla GES was assessed by PCR. Positive isolates to the presence of resistance genes were identified by 16sDNA PCR amplification and sequencing and the resistance genes alelles for bla TEM and bla CTX-M were determined the same way. Only some Enterobacteriaceae (n=6) were found positive for the presence of antimicrobial resistance determinants: qnrB (n=4), which confers low-level resistance to fluroquinolones; bla TEM-1 (n=1), an allele that only confers resistance to early cephalosporins in addition to many penicillins while other bla TEM alleles confer resistance to extended spectrum beta-lactams; and bla OXY (n=2), a chromosomally encoded beta-lactamase that confers resistance to aminopenicillins and carboxypenicillins, found when screening for the presence of bla CTX-M . The qnrB carriers were identified as Citrobacter spp, one Escherichia coli carried bla TEM-1 , one Klebsiella oxytoca isolate carried bla OXY while other carried both bla OXY and qnrB. The collection of isolates studied, despite having the presence of some resistance genes, did not reveal the presence of metallo-beta- lactamases or extended-spectrum- beta-lactamases since the genes found only confer resistance to early cephalosporins and penicillins and low-level resistance to fluorquinolones. The data shows that cockles from Lagoa de Óbidos do not present an excessive risk to the consumer with regards to infection with antimicrobial resistance bacteria. Acknowledgements This work was supported by the Integrated Programme of SR&TD “Smart Valorization of Endogenous Marine Biological Resources Under a Changing Climate” (reference Centro-01-0145-FEDER- 000018), co-funded by Centro 2020 program, Portugal 2020, European Union, through the European Regional Development Fund. Keywords: cockles, Cerastoderma edule, antimicrobial resistance, Fluroquinolones, extended-spectrum-beta-lactamases, Metallo-beta-lactamases, Citrobacter, Klebsiella oxytoca Conference: IMMR'18 | International Meeting on Marine Research 2018, Peniche, Portugal, 5 Jul - 6 Jul, 2018. Presentation Type: Poster Presentation Topic: Blue Biotech Citation: Ribeiro J, Pires P, Costa CF, Baptista TM, Rodrigues A, Quesada A and Campos MJ (2019). Antimicrobial profile of Enterobacteriaceae and Vibrionaceae isolated from Cerastoderma edule.. Front. Mar. Sci. Conference Abstract: IMMR'18 | International Meeting on Marine Research 2018. doi: 10.3389/conf.FMARS.2018.06.00045 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 27 Apr 2018; Published Online: 07 Jan 2019. * Correspondence: PhD. Maria J Campos, Centro de Ciências do Mar e do Ambiente (IPLeiria), ESTM - Polytechnic Institute of Leiria, Peniche, Portugal, mcampos@ipleiria.pt Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Joana Ribeiro Pedro Pires Cátia F Costa Teresa M Baptista Americo Rodrigues Alberto Quesada Maria J Campos Google Joana Ribeiro Pedro Pires Cátia F Costa Teresa M Baptista Americo Rodrigues Alberto Quesada Maria J Campos Google Scholar Joana Ribeiro Pedro Pires Cátia F Costa Teresa M Baptista Americo Rodrigues Alberto Quesada Maria J Campos PubMed Joana Ribeiro Pedro Pires Cátia F Costa Teresa M Baptista Americo Rodrigues Alberto Quesada Maria J Campos Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.