Broadband Homodecoupled Time-Shared H-1-C-13 And H-1-N-15 Hsqc Experiments

The concepts of pure-shift NMR and time-shared NMR are merged in a single experiment. A C-13/N-15 time-shared version of the real-time BIRD-based broadband homodecoupled HSQC experiment is described. This time-efficient approach affords simultaneously H-1-C-13 and H-1-N-15 pure-shift HSQC spectra in a single acquisition, while achieving substantial gains in both sensitivity and spectral resolution. We also present a related C-13/N-15-F2-coupled homodecoupled version of the CLIP-HSQC experiment for the simultaneous measurement of (1)J(CH) and (1)J(NH) from the simplified doublets observed along the direct dimension. Finally, a novel J-resolved HSQC experiment has been designed for the simple and automated determination of both (1)J(CH)/(1)J(NH) from a 2D J-resolved spectrum. (C) 2018 Elsevier Inc. All rights reserved.