Endothelial Hif-1a, Inflammation, Hypertesion and Chronic Kidney Disease

Introduction: Hypertension and chronic kidney disease (CKD) drive each other and they are one of the most prevalent medical conditions with high morbidity and mortality in the United States and worldwide. Emerging evidence indicates that increased inflammatory response is involved in both hypertension and CKD. We and others have recently showed that persistently elevated HIF-1a in particular in endothelial cells contributes to hypertensive models induced by angiotension II, a potent vasoconstrictor. However, the role of endothelial HIF-1α in hypertension and CKD remains unidentified. Hypothesis: To determine the general significance of persistently elevated endothelial HIF-1 in hypertension and CKD, we used LIGHT, a new family member of TNF-α super family-infused hypertensive CKD mouse model. Methods: Six to twelve VE-cadherin cre+ mice and Hif-1αf/fVE-cadherin cre+ mice for each group were infused with LIGHT by minipump (4ng/day) and PBS as controls.. We collected urine for proteinuria analysis and measu...