Peroxisome proliferator activator receptor alpha (PPARα) activation using free fatty acid library; fatty acids compared

Peroxisome proliferator activated receptor alpha (PPARA) is a nuclear hormone receptor, of particular interest for its impact on a number of health and wellness issues, including the circulatory system, wound healing, skin inflammatory issues and barrier function. To assess direct and limited indirect PPARA activation, a reporter assay was developed using a Gal4-PPARA Ligand Binding Domain (LBD) fusion construct and the upstream activating sequence (UAS) controlling luciferase production.