Characterization Of Tunnel Mutants Reveals A Catalytic Step In Ammonia Delivery By An Aminoacyl-Trna Amidotransferase

The Helicobacter pylori Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase (GatCAB) utilizes an uncommonly hydrophilic, similar to 40 angstrom ammonia tunnel for ammonia/ammonium transport between isolated active sites. Hydrophilicity of this tunnel requires a distinct ammonia transport mechanism, which hypothetically occurs through a series of deprotonation and protonation steps. To explore the initiation of this relay mechanism, the highly conserved tunnel residue D185 (in the GatA subunit) was enzymatically and computationally investigated by comparing D185A, D185N, and D185E mutant enzymes to wild-type GatCAB. Our results indicate that D185 acts as an acid/base residue, participating directly in catalysis. To our knowledge, this is the first example of acid/base chemistry in a glutamine-dependent amidotransferase ammonia tunnel.