Protection of 1,2,3,4,6-penta-O-galloyl-β-D-glucose against apoptosis of PC12 cells induced by MPP~+

Objective To study the protection of 1,2,3,4,6-penta-O-galloyl-β-D-glucose(β-PGG) on apoptosis of PC12 cells from Parkinsonu0027s disease models induced by MPP+ and its mechanism. Methods PC12 cells were incubated in high glucose DMEM medium. One week before drug treatment, nerve growth factor was added to the cultures at the final concentration of 50 ng/m L. PC12 cells were divided into control group, MPP+ group, and 50 μmol/L β-PGG groups pretreated for 7, 12, 20, and 30 h. The survivals of PC12 cells in MPP+ were observed after pretreatment with β-PGG for different periods. The death of PC12 cells in MPP+ was evaluated with trypan blue staining method, and the activity of the PC12 cells was determined by MTT assay. The expression of Bax, Fas, Fas L, procaspase-3, procaspase-8, and procaspase-9 was analyzed by Western blotting method, and then the activity of caspase-3, caspase-8, and caspase-9 was examined. Results The death rates of PC12 cells in control group were the lowest, while those in MPP+ group were the highest, and those in β-PGG groups pretreated after 12 h were significantly decreased compared with MPP+group(P 0.01). The activities of the PC12 cells in MPP+ group were the lowest, and those in β-PGG group pretreated for 12 h were further increased, while those in β-PGG group pretreated for 20 h were the highest. The protein contents of Bcl-2, procaspase-3, procaspase-8, and procaspase-9 in β-PGG group pretreated for 5 h were increased, and increased to peak at pretreated for 15 h. On the contrary, protein contents of Bax, Fas, and Fas L in β-PGG group pretreated for 5 h were decreased, and decreased to the minimum at pretreated for 30 h. The activities of caspase-3, caspase-8, and caspase-9 of the PC12 cells in β-PGG group pretreated for 15 h were 36.5%, 40.2%, and 42.2% of those in MPP+ group, respectively. Conclusion β-PGG has protection against apoptosis of PC12 cells induced by MPP+, and its mechanism is related to inhibiting the apoptosis of PC12 cells induced by MPP+ and then increase survival rate of PC12 cells by increasing the expression of Bcl-2, inhibiting the expression of Bax, Fas, and Fas L, and decreasing activities of caspase-3, caspase-8, and caspase-9.