UGT79B31 is responsible for the final modification step of pollen-specific flavonoid biosynthesis in Petunia hybrida

Main conclusion UGT79B31 encodes flavonol 3- O -glycoside: 2″- O -glucosyltransferase, an enzyme responsible for the terminal modification of pollen-specific flavonols in Petunia hybrida . Flavonoids are known to be involved in pollen fertility in petunia ( P . hybrida ) and maize ( Zea mays ). As a first step toward elucidating the role of flavonoids in pollen, we have identified a glycosyltransferase that is responsible for the terminal modification of petunia pollen-specific flavonoids. An in silico search of the petunia transcriptome database revealed four candidate UDP-glycosyltransferase (UGT) genes. UGT79B31 was selected for further analyses based on a correlation between the accumulation pattern of flavonol glycosides in various tissues and organs and the expression profiles of the candidate genes. Arabidopsis ugt79b6 mutants that lacked kaempferol/quercetin 3- O -glucosyl(1 → 2)glucosides, were complemented by transformation with UGT79B31 cDNA under the control of Arabidopsis UGT79B6 promoter, showing that UGT79B31 functions as a flavonol 3- O -glucoside: 2″- O -glucosyltransferase in planta. Recombinant UGT79B31 protein can convert kaempferol 3- O -galactoside/glucoside to kaempferol 3- O -glucosyl(1 → 2)galactoside/glucoside. UGT79B31 prefers flavonol 3- O -galactosides to the 3- O -glucosides and rarely accepted the 3- O -diglycosides as sugar acceptors. UDP-glucose was the preferred sugar donor for UGT79B31. These results indicated that UGT79B31 encodes a flavonoid 3- O -glycoside: 2″- O -glucosyltransferase. Transient expression of UGT79B31 fused to green fluorescent protein (GFP) in Nicotiana benthamiana showed that UGT79B31 protein was localized in the cytosol.