Production of a Mono-Biotinylated EGFR Nanobody in the E. Coli Periplasm Using the Pet22b Vector

Alfiah Noor, Gudrun Walser, Matthijs Wesseling,Philippe Giron, Albert-Menno Laffra, Fatima Haddouchi,Jacques De Grève,Peter Kronenberger

BMC research notes（2018）

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摘要

Our aim was to produce a mono-biotinylated single domain antibody (‘nanobody’) specific for the epidermal growth factor receptor (EGFR), which is overexpressed in many cancer cells. The binding of the nanobody and its function are tested in cancer cells. The construct could be used to carry variable therapeutic or diagnostic load using biotin-streptavidin bridging.

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关键词

Nanocarriers,EGFR,Nanobody,Single-domain antibody,VHH domain,Mono-biotinylation,Streptavidin,Cancer

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