A Method for Single-Stranded Ancient DNA Library Preparation.
Methods in molecular biology (Clifton, N.J.)(2019)
摘要
Genomic library preparation from highly degraded DNA is more efficient when library molecules are prepared separately from the complementary strands of DNA fragments. We describe a protocol in which libraries are constructed from single DNA strands in a three-step procedure: single-stranded ligation of the first adapter with T4 DNA ligase in the presence of a splinter oligonucleotide, copying of the DNA strand with a proofreading polymerase, and blunt-end ligation of the second double-stranded adapter with T4 DNA ligase.
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关键词
Ancient DNA,Library preparation,NGS,Single-stranded ligation,T4 DNA ligase
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