Iatrogenic Pneumopericardium and Cardiac Tamponade after Fiberoptic Bronchoscopy with Transbronchial Biopsy

1053 Objectives The aim of this study was to perform the cytotoxicity study of 188Re-human serum albumin microspheres (188Re-HSAM) in GP7TB cell line. The study was confirmed the inhibition effect of 188Re-HSAM in GP7TB hepatoma cell line. Methods HSA microspheres were synthesized by gradually-heating method. Amino borane-reduced 188ReO4− was interacted with carbon oxide to form [188Re(OH2)3 (CO) 3]+.[188Re(OH2)3(CO)3]+ was employed as a precursor for directly labeling HSA microspheres with 188ReO4−. Cytotoxicity assay was performed using 6-well disks. Individual assay of a 6-well disks were inoculated with 2 ml DMEM medium and 10%FBS containing 1×105 cells. All disks were incubated for 24 h to achieve 60-70% confluency. Administrated 188Re-HSAM and 188Re in serial dilution to each 6-well disks. The dose of 188Re-HSAM and 188Re was 0, 1, 10, 100 and 1000 μCi per assay. GP7TB cells were incubated with 188Re-HSAM and 188Re for 24 hour at 37 degrees. 24 hours later, the cells were washed twice with PBS, and dissolved in 10% trypsin. The numbers of the alive cells were calculated. Results The size of HSA microspheres was distributed between 20~35 μm. The yield of [188Re(CO)3(OH2)3]+ was 75~80%. The labeling efficiency of 188Re-HSAM was more than 85%. The viability of GP7TB cells was 100%, 87%, 81%, 39% and 21% with 188Re-HSAM in 0, 1, 10, 100 and 1000 μCi/assay, respectively. The viability of GP7TB cells was 100%, 101%, 91%, 66% and 30% with 188Re in 0, 1, 10, 100 and 1000 μCi/assay, respectively. The viability of GP7TB cells was 97% with HSAM. Conclusions This study showed that 188Re-HSAM could improve the cytotoxic effect and had the capability of radiotherapy. Research Support 1.Luboldt W, Pinkert J, Matzky C, Wunderlich G, Kotzerke J. Radiopharmaceutical tracking of particles injected into tumors: a model to study clearance kinetics. Curr Drug Deliv. 2009 Jul;6(3):255-60. 2.Schiller E, Bergmann R, Pietzsch J, Noll B, Sterger A, Johannsen B, Wunderlich G, Pietzsch HJ. Yttrium-86-labelled human serum albumin microspheres: relation of surface structure with in vivo stability. Nucl Med Biol. 2008 Feb;35(2):227-32. Epub 2007 Dec 20.