Daily consumption of monoglucosyl-rutin prevents high-fat diet-induced obesity by suppressing gastric inhibitory polypeptide secretion in mice

Background: Alpha monoglucosyl-rutin (4(G)-alpha-D-glucopyranosyl rutin, alpha MR) has been shown to stimulate antioxidant defenses and anti-glycation. We evaluated the effects of aMR on body weight gain in mice. Methods: Male C57BL/6J mice were divided into four groups: Control low-fat diet, low-fat diet + 0.5% alpha MR, high-fat diet, and high-fat diet + 0.5% alpha MR. Blood chemistry, hepatic lipids, and serum metabolic hormones and cytokines were evaluated after 4 and 13 weeks. Results: After 6 weeks, the high-fat diet group gained more weight than the low-fat diet group. Supplementing the high-fat diet with alpha MR suppressed weight gain by week 13. Visceral fat weight was higher in the high-fat diet group on weeks 4 and 13, while alpha MR supplementation inhibited increase on week 13 but not on week 4. Serum levels of gastric inhibitory polypeptide were higher in the high-fat-diet group than in the low-fat-diet group. alpha MR supplementation inhibited this elevation and regulated levels of serum leptin and hepatic triglycerides. Conclusion: For the first time, we demonstrated how daily consumption of alpha MR inhibits diet-induced visceral fat accumulation by regulating the secretion of gastric inhibitory polypeptide, which thereby prevents excess weight gain. Therefore, alpha MR may be a promising potential functional food.