Generation of two transgenic amphioxus lines using the Tol2 transposon system

Amphioxus or lancelets are regarded as a promising model animals for studying developmental mechanisms in chordates, and the evolution of vertebrate characters, because of their important phylogenetic position and their genomic and anatomical simplicity（Bertrand and Escriva, 2011; Holland and Yu, 2004）. Although several recent advances have made amphioxus more tractable as