Actions of Resveratrol and Serotonin Added to Stored Bloods on Lipid Peroxidation and Oxidative DNA Damage

Human blood is stored for a specific time for various uses (1). However, reactive oxygen species (ROS) formation, such as superoxide anion radical (O2), hydroxyl radical (OH.) and H2O2, occurs during storage (2). In addition, erythrocytes of stored blood results in progressive deregulation of the redox poise time dependently, as it is accompanied increased GSSG levels and decreased GSH anabolism. In anaerobic conditions, erythrocytes of stored blood continue to consume glucose, resulting in acidosis and decreased PH. In addition, the decreasing ATP and 2,3 biphosphoglycerate levels of erythrocyte components in stored blood promotes apoptosis and eryptosis time-dependently. Thus, an increase in heme iron of the medium promotion the formation of OH. and O2 radicals with the reactions of Haber Weis and Fenton (3). Consequently, the formed free radicals cause lipid, protein and DNA oxidation, which are all structural membrane components, cellular elements, core proteins and nucleic acids in stored blood (2,4). Therefore, immediately some changes begin to occur in the stored blood cells starting from the initial time of blood storage (1). When there are at least three double bonds in lipid fatty acids, malondialdehyde (MDA) occur as end product in the result of lipid peroxidation (5). MDA quantities in stored blood provides information about free radical damage (6). Therefore, it is important to examine lipid peroxidation in stored blood to assess degree of oxidative damage. ROS does not only affect lipids. Reactive oxygen and nitrogen species can also induce DNA mutations. O2, OH. and peroxynitrite (ONOO) radicals are known to cause many types of DNA damage (7). Among base modifications, 8hydoxy-2-deoxyguanosine (8-OHdG) also forms as a result of guanine residue oxidation. Therefore, 8OHdG serves as a sensitive biomarker of oxidative DNA damage (8). Also, it is known that OH. radicals are also responsible for the formation of 8-OHdG (9). Because mitochondria possess an electron carrying system and lack an RNA polymerase ABSTRACT