Few Layered Graphene Oxide Thin Films: A Potential Matrix for Immunosensors

We have synthesized highly exfoliated graphene oxide (GO) and used it for the fabrication of electrochemical immunosensor. The thin films of GO were spin coated onto ITO/glass substrate and thereafter Bovine Serum Albumin (BSA) antibodies (Ab) were immobilized using EDC-NHS binding chemistry to design immunobioelectrode. The graphene oxide films were characterized using different spectroscopic techniques viz FT-IR, UV-Vis spectroscopy and HRTEM. The electrochemical activity of designed immunoelectrodes was studied by cyclic voltammetry (CV) that revealed a quasi reversible behaviour of graphene oxide films. The differential pulse voltammetry (DPV) measurements of the fabricated immunoelectrodes showed successful detection of analyte at very low concentration ranges (50fg/mL-100ng/mL). The present study entails the potential of GO for the development of various immunosensors for different diseases.