A simple gas pressure manometer for measuring hydrogen production by hydrogenogenic cultures in serum bottles

•The H2 producing capacities of T. maritima, T. kodakarensis and E. cloacae were studied in 116mL-serum-bottle.•A linear relationship was shown between the H2 partial pressure and the sum of these of H2 and CO2 of each microorganisms.•Under some assumptions, determination of H2 partial pressure was achievable using a simple manometer.