Synthesis and Applicability of Base‐Discriminating DNA‐Triplex‐Forming 19F NMR Probes

Phosphoramidite building blocks of CF3‐modified N4(6‐amino‐2‐pyridinyl)deoxycytidines were synthesized, incorporated into triplex‐forming 2′‐deoxyoligonucleotide strands and the applicability of the probes to recognize nucleobase content in the pyrimidine‐rich strand of double‐helical DNA targets was evaluated. As expected, the obtained 19F NMR resonances were sensitive to the base content and unique 19F NMR spectral fingerprints could be obtained.