HISTOPATHOLOGICAL AND IMMUNOHISTOCHEMICAL STUDY OF MALIGNANT LYMPHOMAS

Introduction: The morphologic classification of lymphomas in the field of hematopathology includes different techniques such as immunohistochemistry and genotypic studies in order to appropriately classify and subclassify these entities according to the WHO Classification; so, the patients can receive the best treatment. Objective: The study with immunohistochemical technique was to classify the different anatomopathological entities of these neoplastic lymphoid processes. Material and Methods: We studied 1813 biopsies of different sites diagnosed such as malignant lymphomas at the Pathology Department of the National Oncology Institute, Havana City, Cuba, from the period of 2007 to 2018 with a panel of different antibodies for hematolymphoid processes employing the technique of immunohistochemistry in paraffin blocks. The manual and automated immunohistochemical staining were used. The visualization systems included the Polymer Detection System and the ultraview Universal DAB Detection Kit. Results: The Non-Hodgkin´s B Cell Lymphomas were the most frequent immunophenotypic type (1303 cases)(71,86%).The B Cell Lymphomas CD20 positive were 1184 cases (65,03%).The most frequent histopathological type was the Diffuse Large B Cell Lymphomas (649 cases)(35,79%).The Peripheral T Cell Lymphomas (76 cases) and Anaplastic Large Cell Lymphomas (61 cases) were the most principal types diagnosed in the group of Non-Hodgkin´s T Cell Lymphomas (137 cases in total)(7,55%).The extranodal location were 868 cases (47,87%) and the Head and Neck location was the most common site diagnosed in this group (180 cases) (20,73%). Conclusion: The study demonstrated the different histologic types, locationand frequencies of the lymphomas in our daily work of a period of time of 12 years in the Immunohistochemistry Laboratory at the Pathology Department. Keywords: anaplastic large cell lymphoma (ALCL); diffuse large B-cell lymphoma (DLBCL); peripheral T-cell lymphomas (PTCL).