MP28-18 IMPLEMENTATION OF PROSTATE BIOPSY TISSUE PRINT TECHNOLOGIES FOR MOLECULAR BIOMARKER STUDIES

You have accessJournal of UrologyProstate Cancer: Markers I1 Apr 2017MP28-18 IMPLEMENTATION OF PROSTATE BIOPSY TISSUE PRINT TECHNOLOGIES FOR MOLECULAR BIOMARKER STUDIES Sandra Gaston, Peter Kolettis, James Bryant, Soroush Rais-Bahrami, Jeffrey Nix, Mark DeGuenther, Michael Kearney, George Adams, William Grizzle, and Gary Kearney Sandra GastonSandra Gaston More articles by this author , Peter KolettisPeter Kolettis More articles by this author , James BryantJames Bryant More articles by this author , Soroush Rais-BahramiSoroush Rais-Bahrami More articles by this author , Jeffrey NixJeffrey Nix More articles by this author , Mark DeGuentherMark DeGuenther More articles by this author , Michael KearneyMichael Kearney More articles by this author , George AdamsGeorge Adams More articles by this author , William GrizzleWilliam Grizzle More articles by this author , and Gary KearneyGary Kearney More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2017.02.831AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES With the introduction of multiple tissue-based biomarker tests for prostate cancer (PCa) the allocation of formalin fixed paraffin embedded (FFPE) biopsy tissues has become challenging. This is especially true for patients managed by active surveillance or non-surgical treatment where no radical prostatectomy specimen is available. Our goal is to develop and fully implement tissue print technologies that allow us to obtain high quality RNA and DNA from prostate biopsy cores without compromising the tissue for histopathology and other FFPE based testing. METHODS Biopsy tissue prints (nitrocellulose blots) were obtained from each core during the transfer of the tissue from the cutting needle to the fixation jar and each print was immediately snap-frozen. Tissue prints were processed by a central laboratory to obtain purified RNA and DNA for QC, molecular marker discovery and targeted biomarker assays. RESULTS At our Boston site (private office practice) we have prospectively collected 444 sets of biopsy tissue prints (>5300 cores) from study subjects undergoing standard diagnostic biopsy. Biopsy tissue print collection has also been implemented at two sites in Birmingham (an academic medical center and a private office practice) to support biomarker studies in African American patients and in patients undergoing mpMRI-US fusion guided biopsy. To date we have prospectively collected 273 sets of tissue prints (>2400 cores) from our Birmingham sites. We routinely harvest 50,000-200,000 cells/print. From cores with >50% high grade PCa the yield/print mean, median (SD) for DNA is 1611 ng, 942 ng (1191) and for RNA is 550 ng, 481 ng (506); from cores with no PCa the yield/print for DNA is 1020 ng, 926 ng (744) and for RNA is 351 ng, 250 ng (418). Prostate biopsy tissue print RNA and DNA is snap-frozen quality and has been successfully utilized for gene expression profiling, genotyping, DNA methylation and sequencing analyses. CONCLUSIONS Tissue print technologies provide a practical approach to biopsy-based biomarker analyses that preserves the tissue core for pathology diagnosis and other FFPE based testing. For research studies, prospective collection of biopsy tissue prints is feasible in both academic and private practice settings. Because tissue prints provide high quality RNA and DNA suitable for a wide range of molecular biomarker tests, the technology may also be useful in situations where there is insufficient FFPE biopsy tissue to satisfy clinical molecular testing requirements. © 2017FiguresReferencesRelatedDetails Volume 197Issue 4SApril 2017Page: e345-e346 Advertisement Copyright & Permissions© 2017MetricsAuthor Information Sandra Gaston More articles by this author Peter Kolettis More articles by this author James Bryant More articles by this author Soroush Rais-Bahrami More articles by this author Jeffrey Nix More articles by this author Mark DeGuenther More articles by this author Michael Kearney More articles by this author George Adams More articles by this author William Grizzle More articles by this author Gary Kearney More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...