A New Exploration of Extracting and Purifying the Coenzyme F420 from Natural Sludge

Coenzyme F 420 is one kind of special flavin cofactors which exist in some Archaea and bacteria, it is a low potential electron transfer carrier in methanogens. In this study, we designed three specific primers of F 420 -dependent glucose-6-phos-phate dehydrogenase gene to determine which environment contains the more abundant coenzyme F 420 , and methods for separating, extracting and purifying the coenzyme F 420 from this environmental samples were carried out, the pure coenzyme F 420 was obtained by some optimizing methods from the natural sludge , and the purity and concentration of coenzyme F 420 we got is just as good as the FMN standard sample which confirmed by their fluorescence emission spectrum. These optimizing methods would save more energy and time to get the good quality coenzyme F 420 .