Quantification of pollen mediated gene flow in maize

Sorina Popescu, Oana Ioja-Boldura, Andrei Marutescu, Ioan Sabau,Elena Marcela Badea

ROMANIAN BIOTECHNOLOGICAL LETTERS(2010)

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摘要
One approach to ensuring coexistence of genetically modified (GM) and conventional maize (Zea mays L.) is the understanding of pollen dispersal in order to keep GM-inputs below the regulated tolerance threshold of 0.9%. In defining science-based isolation distances between GM and non-GM maize fields, cross-fertilization rates have been studied both in experimental and commercial fields. Gene flow was followed based on Bt maize sequence information. Our results pointed out that cross-fertilization levels rapidly decrease with increasing distance from the pollen source and a 25 m isolation distance would be sufficient to keep GM-inputs from cross-fertilizations below the tolerance threshold of 0.9% in the harvest of neighboring non-GM maize fields.
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关键词
Bt maize,gene flow,q-PCR
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