Expression of Apoptosis-Related Fas Antigen and Bcl-2 Protein in Fibroblasts Derived from Hypertrophic Scar and Keloid

Objective To investigate the expression of apoptosis-related protein Fas and Bcl-2 and to explore the corresponding mechanism. Methods Six samples of hypertrophic scars and 6 of keloids were collected cultured, and only 6～10 passages fibroblasts were selected for experiment. With the help of the flow cytometer and adherent cell analysis and sorting interactive laser coytometer (ACAS 570), the expression of apoptosis-related protein -Fas and Bcl-2 were detected. Results The expression of Fas antigen in the fibroblasts derived from the keloids were significant elevated in comparison with that in the fibroblasts from hypertrophic scars. The levels of the Bcl-2 protein, loe in both groups, did not differ significantly between the 2 groups, respectively. Conclusions In contrast to that hypertrophic scars from fibroblasts derived from keloids showed high expressed Fas antigen and resistance to apoptosis induced by FasMcab. Considering the low expression of Bcl-2 protein, we assume that Fas receptor on firoblasts derived from keloids may be insert for functioning.