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一株与血红铆钉菇相关的青霉属菌株的分离与鉴定

Yuan-wei MA,Yu LIU,Kang MA,Feng XU,Shou-xian WANG,Lan-qing WANG

Biotechnology(faisalabad)（2014）

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Abstract
目的:鉴定一株从采自北京市房山区的野生血红铆钉菇中分离得到的丝状真菌,分析其遗传地位,为进一步研究该菌株与血红铆钉菇的关系奠定基础.方法:以血红铆钉菇为材料,进行了组织分离,获得1株丝状真菌菌株,编号为JZB2120006,对其进行了形态学鉴定,并提取了基因组DNA,进行了ITS片段的扩增、测序及系统发育分析.结果:分子生物学鉴定结果表明,该菌株与Penicillium sclerotiorum ZZ07-5菌株有99.3％的同源性.结论:结合形态学特征将该菌株鉴定为P.sclerotiorum,ITS基因序列GenBank登录号为KC594044.
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Key words
penicillium sp,strain
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