Simultaneous determination of human plasma concentration of glycyrrhizic acid and matrine by HPLC

Objective To establish an HPLC methods for the simultaneous determination of human plasma concentration of glycyrrhizic acid and matrine.Methods Ultimate AQ-C18(250mm ×4.6mm,5μm)was chosen as the chromatographic column.The column temperature was at room temperature(25℃).The mobile phase was composed of acetonitrile and 0.05mol·L-1 aqueous phosphoric acid(22.5∶77.5,adjusted to pH 7.5with triethylamine).The flow rate was set at 1.0mL·min-1.The detection wavelengths were set at 220and 250nm.The injection volume was 50μL.Results The linear ranges of glycyrrhizic acid ammonium salt and matrine were 2.0-200.0μg·mL-1and 2.0-200.0μg·mL-1,respectively.The average recovery was above 95%; both the intraday RSD and the interday RSD were less than 4%.Conclusions The method is simple,rapid,sensitive and reliable, and could be used for the simultaneous determination of human plasma concentration of glycyrrhizic acid and matrine.