The development of 3D organ-on-a-chip devices

Event Abstract Back to Event The development of 3D organ-on-a-chip devices Hossein Bahmaee1*, Gwendolen C. Reilly2, Cecile M. Perrault3 and Frederik Claeyssens1 1 University of Sheffield, Biomaterials Science and Tissue Engineering Group, Materials Science and Engineering Department, United Kingdom 2 University of Sheffield, Insigneo Institute for in Silico Medicine, Department of Materials Science and Engineering, United Kingdom 3 University of Sheffield, Insigneo Institute for in Silico Medicine, Department of Mechanical Engineering, United Kingdom Introduction: The combination of microfluidic-based technologies with biological cells has grown in the last decade into the field of organ-on-a-chip (OC) devices. These devices show great potential for in-vitro testing of drugs, since current culture models still fail to replicate in-vivo microenvironments [2], and research must thus rely on animal testing for analysis; which often fails to anticipate human responses. The OC devices enable the study of the fundamental relationship between structure, forces and function in biological tissues and organs at the scale of biological cells. These devices could overcome the drawbacks of conventional cell assays by combining surfaces that both mimic the biochemistry, geometry and mechanics of the extracellular environment. OC devices can also mimic crucial dynamic variations such as chemical gradients and mechanical forces, which cells experience in-vivo. However, current OC devices are often lacking a 3D microenvironments to mimic in-vitro environments, required for optimal cell growth and tissue development. The aim of this work is to create an OC device integrated with a 3D scaffold to replicate mechanical and physical parameters of the physiological environment. Materials and Methods: The device consists of two main sections, the fluidic chip and 3D scaffold. The chip was fabricated by soft lithography of polydimethylsiloxane (PDMS), from a mold created by laser-based direct write. Shortly, structures were sketched on CAD software. The stage was programmed to follow the depicted structure, and the laser solidified photo-curable polymer; the surface is then rinsed with methanol, leaving a negative replica of the structure. PDMS is then poured and cured over the mold, creating the fluidic channels. The 3D scaffold is created of polymerised High Internal Phase Emulsion (polyHIPE). These polyHIPEs are 3D foams with interconnectd pores with a porosity >74%. In this study 2-Ethylhexyl acrylate, Isobornyl acrylate, Trimethylolpropane triacrlyate, surfactant (Hypermer B246) was mixed with a photoinitiator to produce a photocurable resin. This resin was mixed with 80% v/v fraction water and stirred to produce the emulsion, which was cured under UV on a pre-made pillar-shaped mold to shape the 3D scaffold. The scaffold and chip are assembled and sealed with oxygen plasma. Results and Discussion: The chip was successfully produced with PDMS. A 3D platform made of PolyHIPE was successfully sealed inside the chamber. The specification of the OC device structure is shown in Figure 1. The pores are interconnected, and the size is around 20 µm. This figure clearly indicates that a 3D scaffold environment, based on polyHIPEs can be integrated in conventional PDMS-based microfluidics platforms. It is envisaged that the polyHIPE will support 3D cell growth within the microfluidic environment. The inlet and outlet of the chip will allow nutrient and waste transfer to ensure the survivability of cells. Additionally, mechanical and chemical stimuli can provided by the OC device. Figure.1: Microfluidic chip design a) CAD design b) microfluidic integrated with 3D scaffold c) SEM image of scaffold d) SEM showing the microporosity of polyHIPE material. Conclusion: In this study a microfluidic cell-based chip is successfully combined with 3D polyHIPE scaffolds. This opens up the opportunity to explore 3D cell culture on a chip. The microfluidic platform also enables the dynamic control of the culture condition chemically and mechanically and prepares a microenvironment that allows development of 3D tissue from cultured cells. References:[1] Ertl, P., D. Sticker, V. Charwat, C. Kasper, and G. Lepperdinger, Lab-on-a-chip technologies for stem cell analysis. Trends in biotechnology, 2014. 32(5): p. 245-253.[2] Huh, D., Y.-s. Torisawa, G.A. Hamilton, H.J. Kim, and D.E. Ingber, Microengineered physiological biomimicry: organs-on-chips. Lab on a chip, 2012. 12(12): p. 2156-2164. Keywords: Biomimetic, in vitro, Lab on a chip, 3D scaffold Conference: 10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016. Presentation Type: Poster Topic: Microdevices: reproducing physiology at microscale Citation: Bahmaee H, Reilly GC, Perrault CM and Claeyssens F (2016). The development of 3D organ-on-a-chip devices. Front. Bioeng. Biotechnol. Conference Abstract: 10th World Biomaterials Congress. doi: 10.3389/conf.FBIOE.2016.01.02072 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 27 Mar 2016; Published Online: 30 Mar 2016. * Correspondence: Dr. Hossein Bahmaee, University of Sheffield, Biomaterials Science and Tissue Engineering Group, Materials Science and Engineering Department, Sheffield, United Kingdom, Email1 Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Hossein Bahmaee Gwendolen C Reilly Cecile M Perrault Frederik Claeyssens Google Hossein Bahmaee Gwendolen C Reilly Cecile M Perrault Frederik Claeyssens Google Scholar Hossein Bahmaee Gwendolen C Reilly Cecile M Perrault Frederik Claeyssens PubMed Hossein Bahmaee Gwendolen C Reilly Cecile M Perrault Frederik Claeyssens Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.