Data On Antigen Recognition Hindrance By Antibodies Covalently Immobilized To Protein G Magnetic Beads By Dimethyl Pimelimidate (Dmp) Cross-Linking
DATA IN BRIEF(2019)
摘要
The data presented herein is related to the article entitled "Trypanosoma cruzi immunoproteome: calpain-like CAP5.5 differentially detected throughout distinct stages of human Chagas disease cardiomyopathy" [1]. Electrophoretic analyses under denaturing and reducing conditions indicate that covalent immobilization of human IgG to Protein G magnetic beads by cross-linking with 50 mM dimethyl pimelimidate hinders the recognition of T. cruzi antigens in immunoprecipitation assays. (C) 2019 The Authors. Published by Elsevier Inc.
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关键词
antigen recognition hindrance,antibodies,magnetic beads,dimethyl pimelimidate,protein,cross-linking
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