Comprehensive analysis of mouse retinal mononuclear phagocytes

Anika Lückoff,Rebecca Scholz,Florian Sennlaub,Heping Xu,Thomas Langmann

Nature Protocols（2017）

引用 44|浏览6

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摘要

This protocol describes how to characterize mouse retinal mononuclear phagocytes in vivo and in situ using scanning laser ophthalmoscopy of microglia, quantitative analysis of Iba1-stained retinal sections, CD11b-based retinal flow cytometry, and qRT–PCR analysis of key microglia markers.

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关键词

Neuroimmunology,Retina,Life Sciences,general,Biological Techniques,Analytical Chemistry,Microarrays,Computational Biology/Bioinformatics,Organic Chemistry

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