Scanning Indels in the 5q22.1 Region and Identification of the TMEM232 Susceptibility Gene That is Associated with Atopic Dermatitis in the Chinese Han Population

Background: Atopic dermatitis (AD) is a chronic inflammatory skin disease. The 5q22.1 region was found to have an association with AD in our previous genome-wide association study (GWAS).Objective: To identify the AD susceptibility gene in 5822.1 and observe its expression in AD tissues.Methods: Suggestive indels from the GWAS data were genotyped in 3013 AD patients and 5075 controls from the Chinese Han population with the SequenomMassArray system. Association, Bayesian and bioinformatics analyses were used to identify possible causal indels and genes in the 5q22.1 region. Immunohistochemistry (IHC) was performed to observe protein expression in the tissues. PLINK 1.07 software was used for all statistical analyses.Results: The genotyping and association analysis showed that six deletions and four SNP5 were associated with AD (P < 0.005). The rs11357450 (P-combined = 7.79E-04, OR = 139, logBayes Factor = 129) deletion located in TMEM232 was identified to be the strongest variant Analysis of the genetic model revealed that the dominant model best described rs11357450 (P = 1.96E-03, OR = 122; 95% CI = 1.07-137). IHC showed that the expression of TMEM232 decreased gradually from the granular layer to the basal layer in AD, but in normal tissues, this trend was reversed. Additionally, positive cytoplasm staining was found in lymphocytes around the blood vessels in AD.Conclusions: The study indicates that TMEM232 in the 5q22.1 region is the causal gene for AD in the Chinese Han population. (C) 2017 The Authors. Published by Elsevier B.V.