Forensic genetic study of 29 Y-STRs in Korean population.

•PPY23 and YPlus showed similar sensitivity, but PPY23 showed higher tolerance to humic acid than YPlus.•The detection rate of unique minor alleles called from male/male mixtures was higher for PPY23 than for YPlus.•Comparing the newly added loci, the mean values of gene diversity for PPY23 and YPlus were 0.6715 and 0.8158, respectively.•The discrimination capacity in the 306 unrelated South Korean males for PPY23 was 0.9837, and that for YPlus was 0.9935.