Leucocyte-bound Apolipoprotein B in the Circulation is Inversely Associated with the Presence of Clinical and Subclinical Atherosclerosis.

Background Atherosclerosis is a pro-inflammatory condition, in which leucocyte activation plays an important role. The interaction between circulating leucocytes and apolipoprotein (apo) B-containing lipoproteins results in pro-inflammatory changes of these cells. We aimed to evaluate the relationship between apo B bound to circulating leucocytes and atherosclerosis.Methods Apo B on circulating leucocytes was measured by flow cytometry in subjects with and without cardiovascular disease (CVD), expressed as mean fluorescent intensity in arbitrary units (au). Carotid intima-media thickness (cIMT) was measured using B-mode ultrasound. Data are given as median (interquartile range).Results A total of 396 subjects were included, of whom 183 had a history of CVD. Compared to subjects without CVD, patients with CVD had lower apo B bound to neutrophils (12.7 au (9.8-16.2) and 14.2 au (10 . 1-17.5), respectively, P = 0.038) and to monocytes (2.5 au (1.7-3.1) and 2.7 (1.9-3.6) au, respectively, P = 0.025). No differences were found for lymphocyte-bound apo B. Neutrophil-and monocyte-bound apo B were inversely correlated with cIMT (Spearman's rho: -0.123, P = 0.017 and -0.108, P = 0.035, respectively). Both monocyte- and neutrophil-bound apo B were inversely associated with different factors related to the metabolic syndrome, such as body mass index, triglycerides and complement C3. There was a positive association between erythrocyte-bound apo B and apo B bound to each of the leucocyte classes, possibly reflecting a similar mechanism. Discontinuation of statins in 54 subjects did not influence leucocyte-bound apo B.Conclusion Unexpectedly, the presence of noninternalized apo B-containing lipoproteins on circulating neutrophil and monocyte membranes may represent a protective mechanism against atherosclerosis.