Genome-scale reconstruction of the Streptococcus pyogenes M49 metabolic network reveals growth requirements and indicates potential drug targets.

•We present the first genome-scale model of the metabolism of Streptococcus pyogenes.•We experimentally tested the model using diverse data sets, e.g. auxotrophy experiments.•The model allows to understand and predict growth requirements of S. pyogenes in detail.•The mechanism of pH adaptation in this organism is studied in detail.