A multinested PCR for detection of the equine piroplasmids Babesia caballi and Theileria equi.

M G Montes Cortés,J L Fernández-García, M Á Habela Martínez-Estéllez

Ticks and Tick-borne Diseases（2019）

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摘要

•Development of a multinested PCR (mn-PCR) to diagnose Equine Piroplasmosis.•Comparison of the mn-PCR vs. the cELISA finding a low agreement for B. caballi.•Age and gender are not risk factors for B. caballi by cELISA and by mn-PCR.•Age and gender are risk factors for T. equi by cELISA, only age using the mn-PCR.•Spanish strains are clustered with Israelian isolates basing on the RAP-1 gene.

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Babesia caballi,Theileria equi,Multinested PCR,cELISA,RAP-1 gene

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