Aestuariimicrobium Soli Sp Nov., Isolated From Farmland Soil, And Emended Description Of The Genus Aestuariimicrobium

A Gram-reaction-positive, catalase-positive, non-spore-forming and short rod- or oval-shaped bacterial strain, designated D6(T), was isolated from farmland soil in Xuancheng, Anhui Province, China. Growth occurred at 4-37 degrees C (optimum, 30 degrees C), at pH 6.5-8.5 (optimum, 7.0) and with 0-7 % (w/v) NaCI (optimum, 0.5 % NaCl). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain D6(T) was most closely related to Aestuariimicrobium kwangyangense DSM 21549(T) (98.47 %), followed by Tessaracoccus rhinocerotis YIM 101269(T) (94.46 %). Strain D6(T) had a cell-wall peptidoglycan based on LL-diaminopimelic acid. MK-9(H4) was the predominant menaquinone. The major fatty acids of strain D6(T) were anteiso-C-15:0, iso-C-15:0 and summed feature 4 (iso-C-17:0, I and/or anteiso-C-17:1 B). The major polar lipids were a lipid, glycolipid and phospholipid. The DNA G+C content was 69.2 mol% and strain D6(T) showed low DNA-DNA relatedness to A. kwangyangense DSM 21549(T) (36.45 +/- 0.42 %). Based on these genotypic and phenotypic data, strain D6(T) represents a novel species in the genus Aestuariimicrobium, for which the name Aestuariimicrobium soli sp. nov. is proposed. The type strain is D6(T) (=KCTC 39995(T)=DSM 105824(T)). An emended description of the genus Aestuariimicrobium is presented.