[Differential Expression Profile of Long Non-Coding RNA in Regressive Scar and Mature Scar].

Objective:To analyze the expression profile of long non-coding RNA (lncRNA) in regressive scar and mature scar.Methods:Hematoxylin-eosin staining and Masson staining were used to examine the histology of regressive scar and mature scar.The technique of IncRNA microarray was used to test the IncRNA expression profile in regressive scar and mature scar. The raw data of lncRNA were pretreated for normalization. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis was further performed. Four lncRNA expressions were validated by quantitative PCR.Results:Compared with mature scar, more fibroblasts and deeper Masson staining color were seen in the regressive scar, a total of 1 275 upregulated more than 3-fold were found in the regressive scar. And 596 down regulated more than seventy percent lncRNA were found in the regressive scar. Pathway analysis indicated that chemokine signaling pathway, TNF signaling pathway, insulin secretion and focal adhesion would play important roles in scar development.Conclusions:lncRNA expression profiles in the regressive scar is markedly different in comparison with the mature scar,revealing that lncRNA may participate in pathophysiological process of scar maturation.