Efficiency Of Gold Nanoparticles Coated With The Antimicrobial Peptide Indolicidin Against Biofilm Formation And Development Of Candida Spp. Clinical Isolates
INFECTION AND DRUG RESISTANCE(2018)
摘要
Background: This article examines the use of a novel nano-system, gold nanoparticles coated with indolicidin (AuNPs-indolicidin), against pathogenic Candida albicans biofilms. Candida species cause frequent infections owing to their ability to form biofilms, primarily on implant devices.Materials and methods: We used an integrated approach, evaluating the effect of AuNPs-indolicidin on prevention and eradication of Candida biofilms formed in multi-well polystyrene plates, with relative gene expression assays. Four biofilm-associated genes (FG1, HWP1, ALS1 and ALS3, and CDR1 and CDR2) involved in efflux pump were analyzed using reverse transcription polymerase chain reaction.Results: Treatment with the nano-complex significantly inhibits the capacity of C. albicans to form biofilms and impairs preformed mature biofilms. Treatment with AuNPs-indolicidin results in an increase in the kinetics of Rhodamine 6G efflux and a reduction in the expression of biofilm-related genes.Conclusion: These data provide a chance to develop novel therapies against nosocomially acquired refractory C. albicans biofilms.
更多查看译文
关键词
antimicrobial activity, anti-biofilm activity, fungal biofilm, drug resistance, fungal infections
AI 理解论文
溯源树
样例
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要