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Fluorescence Lifetime Imaging with Time-Gated Detection of Hyaluronidase Using a Long Lifetime Azadioxatriangulenium (ADOTA) Fluorophore.

Rahul Chib,Sebastian Requena,Mark Mummert,Yuri M. Strzhemechny,Ignacy Gryczynski,Julian Borejdo,Zygmunt Gryczynski,Rafal Fudala

Methods and applications in fluorescence（2016）

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Abstract
A fluorescence lifetime imaging probe with a long lifetime was used in combination with time-gating for the detection of hyaluronidase using hyaluronic acid as the probe template. This probe was developed by heavily labeling hyaluronic acid with long lifetime azadioxatriangulenium fluorophores (ADOTA). We used this probe to image hyaluronidase produced by DU-145 prostate cancer cells.
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Key words
hyaluronidase imaging,fluorescence-lifetime imaging,hyaluronidase detection,azadioxatriangulenium (ADOTA) fluorophore,time-gated detection
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