Gene assembly via one-pot chemical ligation of DNA promoted by DNA nanostructures.
CHEMICAL COMMUNICATIONS(2018)
摘要
Current gene synthesis methods are driven by enzymatic reactions. Here we report the one-pot synthesis of a chemically-ligated gene from 14 oligonucleotides. The chemical ligation benefits from the highly efficient click chemistry approach templated by DNA nanostructures, and produces modified DNA that is compatible with polymerase enzymes.
更多查看译文
关键词
dna nanostructures,gene,one-pot
AI 理解论文
溯源树
样例
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要