Enhanced imaging of lipid rich nanoparticles embedded in methylcellulose films for transmission electron microscopy using mixtures of heavy metals.
Micron(2017)
摘要
•Uranyl acetate/tungsten double stains are proposed for imaging lipid rich nanoparticle in TEM.•Combined with methylcellulose embedment, the technique enhances membrane contrast.•The technique works for liposomes, nanodiscs and bicelles.•The double staining should improve quantification of lipid rich nanoparticles.
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关键词
CL,DMPC,DHPC,EM,LRPN,MC,PE,PG,PTA,STA,UA
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