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Deletion of Psbq ’ Gene in Cyanidioschyzon Merolae Reveals the Function of Extrinsic PsbQ ’ in PSII

Maksymilian Zienkiewicz,Tomasz Krupnik,Anna Drożak,Wioleta Wasilewska,Anna Golke,Elżbieta Romanowska

Plant Molecular Biology（2018）

Cited 8|Views7
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Abstract
We have successfully produced single-cell colonies of C. merolae mutants, lacking the PsbQ’ subunit in its PSII complex by application of DTA-aided mutant selection. We have investigated the physiological changes in PSII function and structure and proposed a tentative explanation of the function of PsbQ’ subunit in the PSII complex.
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Key words
Stable red algae nuclear transformation,DTA toxin,Cyanidioschyzon merolae,Chloramphenicol acetyltransferase,PEG,PsbQ’,PSII mutants,PSII extrinsic protein deletion
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