Development of 24 microsatellite markers for the white nosed coati ( Nasua narica ) using 454 sequencing

The White nosed coati ( Nasua narica ) it is a highly social carnivore inhabiting mainly Neotropical forests playing an important ecological role. A technique based on 454 sequencing of a library highly enriched for microsatellite repeats was used to develop loci for Nasua narica . Twenty-four polymorphic microsatellites were developed and tested as markers in the target species. All markers were genotyped on 18 different individuals from three distinct locations. We observed medium to low genetic variation across most loci (mean number of alleles per locus = 5). Levels of expected heterozygosity across all markers was fairly low (mean H E = 0.326, range 0.05–0.49).