Regulation of Coenzyme A Biosynthesis in the Hyperthermophilic Bacterium Thermotoga Maritima.

ABSTRACT Regulation of coenzyme A (CoA) biosynthesis in bacteria and eukaryotes occurs through feedback inhibition targeting type I and type II pantothenate kinase (PanK), respectively. In contrast, the activity of type III PanK is not affected by CoA. As the hyperthermophilic bacterium Thermotoga maritima harbors only a single type III PanK ( Tm -PanK), here we examined the mechanisms that regulate CoA biosynthesis in this organism. We first examined the enzyme responsible for the ketopantoate reductase (KPR) reaction, which is the target of feedback inhibition in archaea. A classical KPR homolog was not present on the T. maritima genome, but we found a homolog (TM0550) of the ketol-acid reductoisomerase (KARI) from Corynebacterium glutamicum , which exhibits KPR activity. The purified TM0550 protein displayed both KPR and KARI activities and was designated Tm -KPR/KARI. When T. maritima cell extract was subjected to anion-exchange chromatography, the fractions containing high levels of KPR activity also displayed positive signals in a Western blot analysis using polyclonal anti-TM0550 protein antisera, strongly suggesting that Tm -KPR/KARI was the major source of KPR activity in the organism. The KPR activity of Tm -KPR/KARI was not inhibited in the presence of CoA. We thus examined the properties of Tm -PanK and the pantothenate synthetase ( Tm -PS) of this organism. Tm -PS was not affected by CoA. Surprisingly however, Tm -PanK was inhibited by CoA, with almost complete inhibition in the presence of 400 μM CoA. Our results suggest that CoA biosynthesis in T. maritima is regulated by feedback inhibition targeting PanK, although Tm -PanK is a type III enzyme. IMPORTANCE Bacteria and eukaryotes regulate the biosynthesis of coenzyme A (CoA) by feedback inhibition targeting type I or type II pantothenate kinase (PanK). The hyperthermophilic bacterium Thermotoga maritima harbors a single type III PanK ( Tm -PanK), previously considered to be unaffected by CoA. By examining the properties of three enzymes involved in CoA biosynthesis in this organism, we found that Tm -PanK, although a type III enzyme, is inhibited by CoA. The results provide a feasible explanation of how CoA biosynthesis is regulated in T. maritima , which may also apply for other bacteria that harbor only type III PanK enzymes.